Study of the Arcobacter butzleri genome and transcriptome and evaluation of Arcobacteraceae genomic features

Buzzanca, Davide
Faculteit Diergeneeskunde
Vakgroep Pathobiologie, Farmacologie en Bijzondere Dieren
Gezamenlijk doctoraat
Universiteit Turijn en UGent
09/07/2018 – Grugliasco, Italy Master’s degree in plant biotechnology (LM-6) – University of Torino 05/12/2015 – Grugliasco, Italy Bachelor’s degree in Agricultural sciences and technologies (agrobiotechnologies) – University of Torino
Academische graad
Doctor in de diergeneeskundige wetenschappen
Taal proefschrift
Prof. dr. Kurt Houf, Faculty of Veterinary Medicine, University of Gent - Prof. Kalliopi Rantsiou, Prof. Valentina Alessandria, Faculty of Agricultural, forest and food Sciences - University of Torino

Korte beschrijving

The Arcobacteraceae family includes Gram-negative bacteria isolated from different environmental matrices. Species of this family are also present in farm animals, and are regularly isolated from food of animal origin. In particular Arcobacter butzleri and Arcobacter cryaerophilus are considered foodborne pathogens as they have also been isolated from stool of humans with gastrointestinal diseases. The pathogenicity of some Arcobacteraceae species has already been studied using simplified in vitro cell models which were used in the study of adhesion and invasion and cytotoxic effects. The different ability of Arcobacteraceae to survive in different hosts and environments suggest an evolutionary pressure with consequently variation in genome content. Moreover, their different physiological and genomic characteristics have led to the recent proposal to reclassify the species in new genera, which has been criticized due to the lack of biological and clinical significance. In the thesis, the clinically relevant Arcobacteraceae species are studied with a focus on Arcobacter butzleri. The latter aiming to evaluate genomic and transcriptomic information from different strains. This species choice was motivated by its higher prevalence in farm animals, food and clinically human cases. Following a literature review in Chapter 1, Chapter 2 includes a comparative genomic analysis of 32 A. butzleri strains as well as the evaluation of their colonization and invasion capacity in contact with mucus-producing and non-mucus-producing in vitro cell models. This experimental approach was followed to assess possible links between colonization and invasion capacity and genome content. Furthermore, the presence of putative virulence genes and their possible correlation with the source of isolation was examined. The genome analysis was followed by transcriptome analysis (Chapter 3) to evaluate the function of several genes annotated on the A. butzleri genomes. More specifically, the glob


Donderdag 29 september 2022, 09:00
Aula Samev 2, Largo Paolo Braccini 2, 10095 Grugliasco (TO)

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